不同毛色山羊皮肤组织 Agouti 基因 mRNA及其编码 ASIP 差异表达研究

doi:10.11843/j.issn.0366-6964.2014.12.004

Abstract

Abstract:

The differential expression of Agouti gene mRNA and its encoding ASIP in goat skin with white，black and brown was detected by SYBR Green real-time PCR and Western blotting respectively in order to investigate the regulation mechanism of Agouti gene on coat color.The results indicated that the expression of mRNA was white>black>brown.There was significant difference of mRNA expression between white and brown (P<0.05)，and between black and brown (P<0.05).No significant difference of mRNA expression was found between white and black (P>0.05).The ASIP expression was white>brown>black，and the significant difference of ASIP expression was detected between white and black (P<0.05)，no significant difference was found between white and brown，and between brown and black.It could be inferred that there might be different regulatory mechanism of Agouti gene in different skin with different coat color.

CLC Number:

S827.2

ZHANG Tian，ZHANG Li-ying，LI Xiang-long，ZHOU Rong-yan，LI Lan-hui. Study on Differential Expression of Agouti Gene mRNA and Encoding ASIP in Goat Skin with Different Coat Color[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1932-1938.

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References

［1］张细权，李加琪，杨关福.动物遗传标记［M］.北京：中国农业大学出版社，1997.
［2］SCHIBLER L，VAIMAN D，OUSTR Y，et al.Comparative gene mapping：a fine-scale survey of chromosome rearrangements between ruminants and humans［J］.Genome Res,1998，8(9)：901-915.
［3］何孟颉.家兔 MITF 基因同源性、多态性分析及不同时期表达水平研究［D］.扬州:扬州大学，2012.
［4］白小青，王金勇.猪毛色遗传的研究进展［J］.动物科学与动物医学,2004,8(21):32-33.
［5］ARGESON A C,NEISON K K,SIRACUSA L D.Molecular basis of the apheliotropic phenotype of mice carrying the hyper variable yellow(Ahvy) mutation at the Agouti locus［J］.Genetics，1996，142：557-567.
［6］黄劲，莫晓川，李红梅.蛋白质印迹法检测小鼠不同器官中细胞色素 C 的含量［J］.贵阳学院学报：自然科学版，2006，(3)：48-50.
［7］ALBRECHT E，KOMOLKA K，KUZINSKI J，et al.Agouti revisited：transcript quantification of the ASIP gene in bovine tissues related to protein expression and localization［J］.PLoS ONE，2012，7(4)：e35282.
［8］任玉红,杨刚,范瑞文,等.黑素皮质素受体1 MC1R在不同毛色羊驼皮肤组织中的表达与定位研究［J］.畜牧兽医学报,2012,43(7):1049-1055.
［9］MOUNTJOY K G,ROBBINS L S,MORTRUD M T，et al.The cloning of a family of genes that encode the melanocortin receptors ［J］.Science，1992，257：1248-1251.
［10］HALL A M,ORLOW S J.Degradation of tyrosinase induced by phenylthiourea occurs followinggolgi maturation［J］.Pigment Cell Res，2005，18(2)：122-129.
［11］SLOMINSKI A,TOBIN D J,SHIBAHARA S，et al.Melanin pigmentation in mammalian skin and its hormonal regulation［J］.Physiol Rev，2004，84(4)：1155-1228.
［12］OLLMANN M M,BARSHG S.Down-regulation of melanocortin receptor signaling mediated by the amino terminus of Agouti protein in Xenopus melanophores［J］.J Biol Chem，1999，274(22)：15837-15846.
［13］SUZUKI I,TADA A,OLLMANN M M，et al.Agouti signaling protein inhibits melanogenesis and the response of human melanocytes to α-melanotropin［J］. J Invest Dermatol，1997，108(6)：838-842.
［14］FONTANESI L，DALL’OLIO S，BERETTI F，et al.Coat colors in the Massese sheep breed are associated with mutations in the Agouti signalling protein (ASIP) and melanocortin 1 receptor (MC1R) genes［J］.Animal，2011，5(01)：8-17.
［15］姜维.太行黑山羊毛囊生长—退行期皮肤组织差异表达筛选与鉴定［D］.杨凌：西北农林科技大学，2012.

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Study on Differential Expression of Agouti Gene mRNA and Encoding ASIP in Goat Skin with Different Coat Color

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